Biotin is required for the fatty acids and glucose formation so that essential energy is produced. Biotin also assists in the metabolism of fats, proteins and carbohydrates. Biotin is available in food naturally and the dietary sources of biotin comprise of brewer’s yeast, cauliflower, bananas, egg yolks, salmon, liver, sardines, mushrooms and legumes. The deficiency of biotin is uncommon. The other possibilities for having biotin as deficiency is genetic biotin deficiency, surgical stomach removal, excessive consumption of alcohol and infant dermatitis. Biotin deficiency leads to hair loss, skin rash, heart problems and high cholesterol. Biotin supplements are effective for eczema, acne, diaper rash, brittle nails and hair loss. People having a history of seizures should avoid using biotin. This is a B vitamin that helps in converting food to energy. It is mandatory that minimum 30 mg is taken daily as there are adequate health benefits. Normal biotin intake strengthens fingernails and also controls blood sugar levels.

Chromium is a natural trace mineral in human nutrition and is needed for proper metabolism of sugar in humans. It is vital for metabolism of fats and carbohydrates. It helps move blood sugar from the bloodstream into cells to be utilized as energy and turn fats, carbohydrates, and proteins into energy. Chromium helps stimulate fatty acid and cholesterol synthesis, which are needed for brain function and other body processes. It activates numerous enzymes that are required to drive many chemical reactions essential to life. Chromium supplements also improve glucose tolerance in people with Turner's syndrome, a disease associated with glucose intolerance. It is also vital for insulin metabolism. It regulates hunger and decreases cravings.

L-Isoleucine- The second of three branched chain amino acids it is important in blood sugar regulation and producing hemoglobin. After exercise, it is responsible for muscle recovery and important in the metabolism to produce energy.

L-Leucine is useful in conjunction with L-Valine and L-Isoleucine as an amino acid supplement. L-Leucine is an amino acid that a body cannot produce on its own. It is expected to be received externally from food sources such as brown rice and nuts or even through some of the dietary supplementation. L-Leucine is showing numerous advantages in the athletic qualities. Within six week of training with L-Leucine in association, a person is able to maximize the aerobic power. In the six weeks the strength and power in the upper body increases. However, research and studies is limited carried on the athletic performance. L-Leucine incorporated into the muscle protein in continuation with two weeks exercise regime will give adequate resistance and the muscle protein synthesis will certainly show an increase. L-Leucine is a preservative of the muscles, but is as well a supplement that enhances the capabilities of muscle building owing to the enhanced protein synthesis rate.

L-Valine is an essential alpha amino acid and one of the three essential branched chain amino acids found in the human body. The other two essential BCAAs are leucine and isoleucine. The name is derived from the plant valerian. Valine is also classified as nonpolar and is known by sickle cell disease sufferers as the substitute amino acid for the glutamic acid, whereby the hydrophobic qualities of valine cause the hemoglobin to not fold correctly. Just like the other three BCAAs, the primary source of valine is through ingestion of proteins, where valine is a component; some such sources would be fish, peanuts, poultry, cottage cheese, lentils and sesame seeds. L-Valine is found in large quantities in muscles, whereby in the muscle metabolism it is essential for the restoration and tissue growth.

Vitamin B12- Also called Riboflavin, B2 is a water absorbed vitamin of which the body must replenish its supplies each day as it cannot be stored. It is necessary for the production of red blood cells and, like the other B vitamins, it works at supporting it supports energy production by helping in the metabolism of various foods.B2 is required for healthy skin as well as hair growth, the nails and has a role in regulating the activity of the thyroid gland. It is also necessary in order that the body can process amino acids found in proteins and fats and to activate vitamin B6 and folic acid.

Vitamin B6contains pyridoxal phosphate, or PLP for short, as the active ingredient and is a vitamin belonging to the B complex grouping. It is functioning mainly as a co-enzyme with others in the human body, aiding in the proper function of several metabolic processes. Neurotransmitter synthesis, as well as hemoglobin and histamine synthesis are just some of the functions of PLP, hemoglobin function, gene expression and aiding in the facilitation of decarboxylation, elimination and other reactions, mainly taking place in the liver. Vitamin B6 was found to diminish by 50% the risk of Parkinson’s disease. Alternative medicine suggests the use of Vitamin B6 to combat autism, in combination with magnesium. This combination has shown effects with children suffering from attention deficit disorders. PLP also seems to show therapeutic effects for diabetic nephropathy.

Zinc, a mineral needed for cell division and repair as well as proper functioning of the immune system. Zinc is also an important antioxidant that neutralizes free radicals in the body.